학술논문
LncRNA profiling of skeletal muscles in Large White pigs and Mashen pigs during development.
Document Type
Article
Author
Source
Subject
*SWINE genetics
*LINCRNA
*POLYMERASE chain reaction
*GENETIC regulation
*NUCLEOTIDE sequence
*SKELETAL muscle
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Language
ISSN
0021-8812
Abstract
Long noncoding RNA (lncRNA) has been increasingly implicated in the regulation of muscle development. Large White pigs have a higher muscle growth rate than do Mashen pigs. In the present study, the lncRNA expression profiles in skeletal muscle of these 2 pig breeds were compared at 1, 90, and 180 d of age using RNA sequencing. We obtained 2,718 million clean reads and identified a total of 5,153 novel lncRNA. We found 1,407 differentially expressed lncRNA that showed consistent expression patterns between the 2 breeds at all the 3 sampling points. Ten lncRNA were randomly selected, and their expression was validated using Real-time Quantitative PCR. In summary, this study identifies a number of lncRNA that correlate with muscle growth. The regulation and function of these lncRNA in muscle growth and development need to be further explored. [ABSTRACT FROM AUTHOR]