학술논문
A kinetic method for distinguishing whether an enzyme has one or two active sites for two different substrates.
Document Type
Article
Source
Subject
*DIGESTIVE organs
*ENZYMES
*ABDOMEN
*BILIARY tract
*AMINES
*AMINO acids
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Language
ISSN
0014-2956
Abstract
We have elaborated a kinetic method which allows us to evaluate whether a Michaelis-Menten-type enzyme acting on two different substrates has one or two active sites. This method has been used with the rat liver L- threonine dehydratase, which catalyzes the dehydrative deamination of both serine and threonine. The experimental data can be fitted to the theoretical plot obtained for the case of a single active site. [ABSTRACT FROM AUTHOR]