부산대학교 도서관

Simple Summary: Alpacas (Vicugna pacos) and llamas (Lama glama) constitute the most significant livestock wealth of the Andean populations of South America. Infectious diseases, particularly respiratory and enteric infections, cause high morbidity and mortality in offspring and adult animals. In this study, we demonstrated that multiple variants of the coronavirus co-circulated among Peruvian alpacas. We also demonstrated that some of these variants bear similarities to coronavirus strains detected in bats. For a better understanding of the infections that afflict these animals, continuous surveillance is needed to identify the emergence of new genotypes and viral variants that are potentially pathogenic to alpacas and humans. Future studies should include the sequencing of genes encoding CoV spike proteins and host receptors to confirm interspecies transmission. Coronaviruses (CoVs) infect a wide range of hosts, including humans, domestic animals, and wildlife, typically causing mild-to-severe respiratory or enteric disease. The main objective of this study was to identify CoV genera and subgenera detected in Peruvian alpacas. Lung lavage specimens were collected from 32 animals aged 1 to 6 weeks. CoVs were identified by using RT-PCR to amplify a pan-CoV conserved region of the RNA-dependent RNA polymerase-encoding gene. A nested PCR was performed to identify β-CoVs. Then, β-CoV-positive samples were subjected to RT-PCR using specific primers to identify the Embecovirus subgenus. Out of 32 analyzed samples, 30 (93.8%) tested positive for at least one CoV genus. β-, α-, or unclassified CoVs were identified in 24 (80%), 1 (3.3%), and 1 (3.3%) of the positive samples, respectively. A CoV genus could not be identified in two (6.7%) samples. A mixture of different CoV genera was detected in two (6.7%) samples: one was co-infected with β- and α-CoVs, and the other contained a β- and an unclassified CoV. A sequence analysis of the amplicons generated by the PCR identified 17 β-CoV strains belonging to the subgenus Embecovirus and two α-CoV strains belonging to Decacovirus. A phylogenetic analysis of two strains revealed a relationship with an unclassified Megaderma BatCoV strain. A subgenus could not be identified in nine β-CoV samples. Our data show a high prevalence and a high genetic diversity of CoV genera and subgenera that infect alpacas, in which the β-CoV subgenus Embecovirus predominated. Our data also suggest a new role for bats in the dissemination and transmission of uncommon CoVs to alpacas raised in rural Peru. [ABSTRACT FROM AUTHOR]