학술논문
Diagnostic accuracy of VIKIA® Rota‑Adeno and Premier™ Rotaclone® tests for the detection of rotavirus in Niger.
Document Type
Article
Author
Source
Subject
*ROTAVIRUSES
*REVERSE transcriptase polymerase chain reaction
*LABORATORIES
*ENZYME-linked immunosorbent assay
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Language
ISSN
1756-0500
Abstract
Objective: We conducted a parallel evaluation of the diagnostic accuracy of VIKIA® Rota-Adeno, a rapid diagnostic test (RDT) and Premier™ Rotaclone® an enzyme immunoassay (EIA) using reverse transcription polymerase chain reaction (RT-PCR) as the reference standard. The study was part of a rotavirus surveillance project in Niger. Results: The sensitivity of both tests was 80.7%. After exclusion of one indeterminate result by visual reading, the specificity of the Premier™ Rotaclone® was 100% by visual or optical density readings and that of VIKIA® Rota-Adeno test was 95.5%. Inter-reader agreement was excellent for both tests (kappa = 1). Our results showed almost similar performance of the EIA and RDT when compared to RT-PCR. Hence, the VIKIA® Rota-Adeno could be a good alternative for use in peripheral health centres where laboratory capacity is limited. [ABSTRACT FROM AUTHOR]