학술논문
Live-cell dSTORM with SNAP-tag fusion proteins.
Document Type
Article
Author
Source
Subject
*IMAGING systems in biology
*CELL lines
*MICROSCOPICAL technique
*HISTONES
*EUKARYOTIC cells
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Language
ISSN
1548-7091
Abstract
The article reports on the study of the live-cell direct stochastic optical reconstruction microscopy (dSTORM) of core histone H2B proteins in different eukaryotic cell lines using SNAP-tags fusion protein. The result shows that cell line-dependent differences in specificity and efficiency of labeling had introduced a very efficient method to minimize nonspecific adsorption of fluorophores. The study also demonstrates how radical anions enable precise control of the number of fluorophores.