부산대학교 도서관

Meiotic chromosome architecture called 'axis-loop structures' and histone modifications have been shown to regulate the Spo11-dependent formation of DNA double-strand breaks ( DSBs) that trigger meiotic recombination. Using genome-wide chromatin immunoprecipitation ( Ch IP) analyses followed by deep sequencing, we compared the genome-wide distribution of the axis protein Rec8 (the kleisin subunit of meiotic cohesin) with that of oligomeric DNA covalently bound to Spo11, indicative of DSB sites. The frequency of DSB sites is overall constant between Rec8 binding sites. However, DSB cold spots are observed in regions spanning ±0.8 kb around Rec8 binding sites. The axis-associated cold spots are not due to the exclusion of Spo11 localization from the axis, because Ch IP experiments showed that substantial Spo11 persists at Rec8 binding sites during DSB formation. Spo11 fused with Gal4 DNA binding domain ( Gal4 BD- Spo11) tethered in close proximity (≤0.8 kb) to Rec8 binding sites hardly forms meiotic DSBs, in contrast with other regions. In addition, H3 K4 trimethylation ( H3 K4me3) remarkably decreases at Rec8 binding sites. These results suggest that reduced histone H3 K4me3 in combination with inactivation of Spo11 activity on the axis discourages DSB hot spot formation. [ABSTRACT FROM AUTHOR]