부산대학교 도서관

Simple Summary: The efficacy of vaccination against type 2 porcine circoviruses is influenced by the level of maternally derived antibodies present at the time of vaccination of the piglets. This aspect was investigated by using two types of vaccines either comprised of a whole virus or its single protein. Both vaccines were able to overcome the impact of the maternal antibodies, however, the immunity, measured by serum antibody levels, developed faster for the whole virus type vaccine. The correlation between the maternally derived antibody levels at the time of vaccination and the challenge viral load showed differences amongst the tested lymph nodes. Additionally, it was confirmed also in this study that group oral fluid samples provide a reliable and relatively easy animal welfare-friendly way of estimating the PCV2 status of groups of pigs. Piglets from a porcine circovirus type 2 (PCV2) stable farm of low and high levels of maternally derived antibodies (MDA) against PCV2 were vaccinated either with a whole virus type or a PCV2 ORF2 antigen-based commercial subunit vaccine at three weeks of age. Two non-vaccinated groups served as low and high MDA positive controls. At four weeks post vaccination, all piglets were challenged with a PCV2d-2 type virus strain and were checked for parameters related to vaccine protection over a four-week observation period. MDA levels evidently impacted the outcome of the PCV2d-2 challenge in non-vaccinated animals, while it did not have a significant effect on vaccine-induced protection levels. The humoral immune response developed faster in the whole virus vaccinates than in the subunit vaccinated pigs in the low MDA groups. Further, high MDA levels elicited a stronger negative effect on the vaccine-induced humoral immune response for the subunit vaccine than for the whole virus vaccine. The group-based oral fluid samples and the group mean viraemia and faecal shedding data correlated well, enabling this simple, and animal welfare-friendly sampling method for the evaluation of the PCV2 viral load status of these nursery piglets. [ABSTRACT FROM AUTHOR]