학술논문
Pentafluorophenyl Ester-FunctionalizedPhosphorylcholinePolymers: Preparation of Linear, Two-Arm, and Grafted PolymerâProteinConjugates.
Document Type
Article
Author
Source
Subject
*GRAFT copolymers
*BIOCONJUGATES
*PHARMACOKINETICS
*HIGH performance liquid chromatography
*LYSOZYMES
*PHENYL compounds
*ADDITION polymerization
*ESTERS
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Language
ISSN
1525-7797
Abstract
Novel pentafluorophenyl (PFP)-ester-functionalized phosphorylcholine(PC) polymers of different architectures were prepared and conjugatedto lysozyme as a model protein. Linear and two-arm poly(2-methacryloyloxyethylphosphorylcholine) (polyMPC) structures containing PFP functionalityat the chain-end were prepared by atom transfer radical polymerization(ATRP) from novel initiators. Additional conjugates were preparedfrom phosphorylcholine-substituted cyclooctene (PC-COE) polymers containingPFP-ester bearing comonomers. The polymerâprotein conjugateswere characterized by HPLC, FPLC, and DLS and were seen to retainmost (â¼80% or greater) of their native enzymatic activity.Pharmacokinetic profiles of the polymerâprotein conjugateswere studied in mice and found to increase the circulation half-lifecompared with lysozyme alone. [ABSTRACT FROM AUTHOR]