부산대학교 도서관

To prevent a decrease in the speed of the biotechnological process and achieve an optimal yield of the target product of biosynthesis (biomass), it is necessary to develop optimal technological parameters for the cultivation of production yeast Saccharomyces cerevisiae for fermentation of high-concentration grain wort. An increase in the concentration of dry substances of fermented wort and alcohol in brews leads to a slowdown in the processes of yeast cell synthesis and fermentation. Therefore, it is necessary to investigate the influence of the technological parameters of the cultivation of production yeast for the synthesis of the required concentration of yeast cells with high biochemical activity. Cultivation of industrial yeast was performed by an osmophilic thermotolerant race of alcoholic yeast Saccharomyces cerevisiae DO-16 (IMB Y - 5099) at a temperature of 30, 32, 35 and 37°C. The initial dry matter concentration was 20, 24 and 28%, the amine nitrogen concentration was 0.5, 0.7 and 0.9 g/dm³. To select the technological parameters of the process of cultivation of industrial yeast for fermentation of high concentrations of wort and to establish the patterns of change, laboratory experimental studies of samples according to the developed technology were conducted. This paper investigates the degree of influence on the synthesis of yeast cells of various process parameters in their interaction, the establishment of multifactor dependence, using the methods of mathematical planning of the experiment. Cultivation of industrial alcoholic yeast Saccharomyces cerevisiae DO-16 was performed in periodic culture. As factors of variation are being used: the initial concentration of dry matter of the wort, the concentration of amine nitrogen, the cultivation temperature. The created mathematical model makes it possible to calculate the concentration of production yeast depending on the initial wort concentration, amine nitrogen concentration and fermentation temperature. According to the equations of the mathematical model, the process of fermentation of high-concentration wort for bioethanol production was optimized. Using Origin software, we found the optimal values of technological parameters of yeast cell biosynthesis: dry matter concentration of grain wort 28%, cultivation temperature 30–32°C, amine nitrogen concentration 0.7 g/dm³. [ABSTRACT FROM AUTHOR]