학술논문
Super-resolved 3D-STED microscopy identifies a layer-specific increase in excitatory synapses in the hippocampal CA1 region of Neuroligin-3 KO mice.
Document Type
Article
Source
Subject
*NEURAL circuitry
*AUTISM spectrum disorders
*SYNAPSES
*NEURAL transmission
*STIMULATED emission
*NEUROPLASTICITY
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Language
ISSN
0006-291X
Abstract
The chemical synapse is one type of cell-adhesion system that transmits information from a neuron to another neuron in the complex neuronal network in the brain. Synaptic transmission is the rate-limiting step during the information processing in the neuronal network and its plasticity is involved in cognitive functions. Thus, morphological and electrophysiological analyses of synapses are of particular importance in neuroscience research. In the current study, we applied super-resolved three-dimensional stimulated emission depletion (3D-STED) microscopy for the morphological analyses of synapses. This approach allowed us to estimate the precise number of excitatory and inhibitory synapses in the mouse hippocampal tissue. We discovered a region-specific increase in excitatory synapses in a model mouse of autism spectrum disorder, Neuroligin-3 KO, with this method. This type of analysis will open a new field in developmental neuroscience in the future. • Super-resolved 3D-STED microscopy can detect individual synapses in the brain tissue. • Neuroligin-3 KO mouse shows no change in synapse numbers in hippocampal CA1 SR. • Neuroligin-3 KO mouse shows an increase in excitatory synapses in hippocampal CA1 SO. [ABSTRACT FROM AUTHOR]