학술논문
Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality.
Document Type
Article
Author
Source
Subject
*MESSENGER RNA
*CURRENT good manufacturing practices
*CHIMERIC antigen receptors
*CELLULAR therapy
*REGULATORY compliance
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Language
ISSN
2045-2322
Abstract
Process development for transferring lab-scale research workflows to automated manufacturing procedures is critical for chimeric antigen receptor (CAR)-T cell therapies. Therefore, the key factor for cell viability, expansion, modification, and functionality is the optimal combination of medium and T cell activator as well as their regulatory compliance for later manufacturing under Good Manufacturing Practice (GMP). In this study, we compared two protocols for CAR-mRNA-modified T cell generation using our current lab-scale process, analyzed all mentioned parameters, and evaluated the protocols' potential for upscaling and process development of mRNA-based CAR-T cell therapies. [ABSTRACT FROM AUTHOR]