학술논문
Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats.
Document Type
Article
Author
Source
Subject
*ASPARTIC acid metabolism
*PROTEIN analysis
*ADENOSINE triphosphate
*ANIMAL experimentation
*BIOLOGICAL models
*CELL separation
*ENZYME-linked immunosorbent assay
*IMMUNOGLOBULINS
*LIQUID chromatography
*MASS spectrometry
*RATS
*RETINA
*WESTERN immunoblotting
*OCULAR hypertension
*DISEASE complications
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Language
ISSN
2090-004X
Abstract
Purpose. To investigate the effect of ocular hypertension-induced isomerization of aspartic acid in retinal proteins. Methods. Adult Wistar rats with ocular hypertension were used as an experimental model. D-β-aspartic acid-containing proteins were isolated by SDS-PAGE and western blot with an anti-D-β-aspartic acid antibody and identified by liquid chromatography-mass spectrometry analysis. The concentration of ATP was measured by ELISA. Results. D-β-aspartic acid was expressed in a protein band at around 44.5 kDa at much higher quantities in the retinas of rats with ocular hypertension than in those of normotensive rats. The 44.5 kDa protein band was mainly composed of α-enolase, S-arrestin, and ATP synthase subunits α and β, in both the ocular hypertensive and normotensive retinas. Moreover, increasing intraocular pressure was correlated with increasing ATP concentrations in the retinas of rats. Conclusion. Ocular hypertension affected the expression of proteins containing D-β-aspartic acid, including ATP synthase subunits, and up-regulation of ATP in the retinas of rats. [ABSTRACT FROM AUTHOR]