학술논문
A novel tdTomato transgenic mouse model to visualize FAP‐positive cancer‐associated fibroblasts.
Document Type
Article
Author
Wei, Feng; Uchihara, Tomoyuki; Yonemura, Atsuko; Yasuda‐Yoshihara, Noriko; Yasuda, Tadahito; Semba, Takashi; Fukuda, Masahiro; Akiyama, Takahiko; Kitamura, Fumimasa; Bu, Luke; Hu, Xichen; Fu, Lingfeng; Zhang, Jun; Kariya, Ryusho; Yamasaki, Juntaro; Aihara, Kazuki; Yamashita, Kohei; Nagano, Osamu; Okada, Seiji; Baba, Hideo
Source
Subject
*TRANSGENIC mice
*MICE
*FIBROBLASTS
*LABORATORY mice
*ANIMAL disease models
*CANCER cells
*STOMACH cancer
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Language
ISSN
1742-464X
Abstract
Fibroblast activation protein (FAP) generally shows low or undetectable expression in most normal tissues but is highly expressed in fibroblasts in almost all carcinomas. FAP is one of the potential molecules to detect activated fibroblasts and has multiple roles in tumour progression. We generated transgenic mice that specifically expressed tdTomato along with FAP promoter activity. Coculturing a mouse gastric cancer cell line and FAP‐tdTomato transgenic mouse‐derived fibroblasts showed that tdTomato expression was elevated in the cocultured fibroblasts. Moreover, stomach wall transplanted tumours in mice also showed FAP‐tdTomato expression in fibroblasts of the stomach and each metastatic legion. These results indicated that FAP‐tdTomato expression in fibroblasts was elevated by stimulation through the interaction with cancer cells. Functionally, collagen production was increased in FAP/tdTomato‐positive fibroblasts cocultured with mouse cancer cells. These FAP‐tdTomato transgenic mice have the potential to be used to investigate real‐time FAP dynamics and the importance of FAP expression in tumour development. [ABSTRACT FROM AUTHOR]