학술논문
Myosin denaturation in heated myofibrils of scallop adductor muscle.
Document Type
Article
Source
Subject
*ADENOSINE triphosphatase
*MYOSIN
*MYOFIBRILS
*ETHYLENEDIAMINETETRAACETIC acid
*SCALLOPS
*MOLLUSKS
*HYDROGEN-ion concentration
*SMOOTH muscle
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Language
ISSN
0919-9268
Abstract
The thermal inactivation of Ca ATPase of scallop myofibrils (0.1 M KCl, pH 7.5) was found to be unaffected by the presence of Ca. Monomeric myosin content and salt solubility decreased much faster than Ca ATPase inactivation in both Ca and EDTA media, which was well explained by faster denaturation of the rod portion than subfragment-1 of myosin. In contrast, when the myofibrils were heated at 0.5 M KCl, a slow decrease in salt solubility was observed, which was also explained by slow denaturation of the rod portion of myosin. Myofibrils from scallop smooth muscle showed the same denaturation pattern as those from adductor muscle. These results show that mollusk myosin is not always stabilized by Ca. [ABSTRACT FROM AUTHOR]