학술논문
Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies.
Document Type
Article
Author
Sharma, Harshita; Kinoshita, Yasunori; Fujiu, Seiichi; Nomura, Shota; Sawada, Mizuho; Ahmed, Shamim; Shibuya, Masaki; Shirai, Kosaku; Takamatsu, Syota; Watanabe, Tsuyoshi; Yamazaki, Hitoshi; Kamiyama, Ryohei; Kobayashi, Tetsuya; Arai, Hidenao; Suzuki, Miho; Nemoto, Naoto; Ando, Ki; Uchida, Hidekazu; Kitamura, Koichiro; Takei, Osamu
Source
Subject
*HUMAN microbiota
*MICROARRAY technology
*LYSOZYME crystallography
*MICROFLUIDICS
*GENE expression
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Language
ISSN
1472-6750
Abstract
Background: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip. Results: In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications. Conclusions: The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies. [ABSTRACT FROM AUTHOR]