학술논문
Radical probing of spliceosome assembly.
Document Type
Article
Source
Subject
*SPLICEOSOMES
*MOLECULAR self-assembly
*HYDROXYL group
*ORGANIC synthesis
*NUCLEOTIDE sequence
*RNA-protein interactions
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Language
ISSN
1046-2023
Abstract
Here we describe the synthesis and use of a directed hydroxyl radical probe, tethered to a pre-mRNA substrate, to map the structure of this substrate during the spliceosome assembly process. These studies indicate an early organization and proximation of conserved pre-mRNA sequences during spliceosome assembly. This methodology may be adapted to the synthesis of a wide variety of modified RNAs for use as probes of RNA structure and RNA-protein interaction. [ABSTRACT FROM AUTHOR]