부산대학교 도서관

The present work describes the development of molecular iodine mediated facile and sensitive method for the spectrophotometric estimation of tolfenamic acid in pharmaceutical and veterinary formulations. Here, in the presence of iodine, the secondary amine of tolfenamic acid coordinates with the lone pair of available electrons with iodine, which results in the formation of hyper iodine complex of tolfenamic acid. The newly formed colored ion complex showed the absorbance maximum at 359 nm. The method was validated as recommended in ICH guidelines. The method was linear over the concentration range of 0.01–0.07 µg/mL (r2 = 0.9998) with the limits of detection and quantitation of 0.002 and 0.006 µg/mL, respectively. Intra-day (mean recovery of 99.7%) and inter-day (mean recovery of 100.9%) method precision was determined. The mean recovery of the accuracy study is between 99.0–100.5%. The method was found to be robust and suitable for assay of tolfenamic acid in pharmaceutical and veterinary formulations. [ABSTRACT FROM AUTHOR]