부산대학교 도서관

The present study deals with the use of cell-free supernatant of bacteria Morganella morganii for synthesizing copper nanoparticles and analysing its larvicidal activity on mosquito larvae. A colour change from blue to pickle green specifies the synthesis production of CuNPs. The nanoparticles were characterized using ultraviolet–visible spectrophotometry, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). XRD pattern shows the major peaks, viz. (111), (200) and (220). SEM analysis shows that nanoparticles are in a spherical shape with a small percentage of elongated particles and size of about 13.5 ± 0.6 nm. A UV–Vis absorption peak was observed at 540 nm. FTIR analysis of nanoparticles exhibits functional groups such as aromatics, alkanes, ethers and alkyl halides. In EDaX (energy-dispersive X-ray) analysis, the peak signal confirms the presence of copper atoms that bound to the synthesized nanoparticles. The synthesized copper nanoparticles were tested for larvicidal efficacy at different time intervals. The above result shows that the copper nanoparticles produce increased toxicity in a time- and dose-dependent manner. An antibacterial and antifungal activity was tested against clinical pathogens, and the highest zone of inhibition was found to be in E. coli, B. subtilis, A. niger, M. anisopliae and Verticillium sp. This study shows that CuNPs possess a good antimicrobial and insecticidal activity which can be explored for commercial purpose. [ABSTRACT FROM AUTHOR]