부산대학교 도서관

The aim of this study was to investigate acetylcholinesterase (AChE) in total blood and liver tissue; butyrylcholinesterase (BChE) in serum and liver tissue; adenosine deaminase (ADA) in serum and liver tissue; and pyruvate kinase (PK) in liver tissue of rats experimentally infected by Fasciola hepatica. Animals were divided into two groups with 12 animals each, as follows: group A (uninfected) and group B (infected). Samples were collected at 20 (A 1 and B 1; n = 6 each) and 150 (A 2 and B 2 ; n = 6 each) days post-infection (PI). Infected animals showed an increase in AChE activity in whole blood and a decrease in AChE activity in liver homogenates ( P < 0.05) at 20 and 150 days PI. BChE and PK activities were decreased ( P < 0.05) in serum and liver homogenates of infected animals at 150 days PI. ADA activity was decreased in serum at 20 and 150 days PI, while in liver homogenates it was only decreased at 150 days PI ( P < 0.05). Aspartate aminotransferase and alanine aminotransferase activities in serum were increased ( P < 0.05), while concentrations of total protein and albumin were decreased ( P < 0.05) when compared to control. The histological analysis revealed fibrous perihepatitis and necrosis. Therefore, we conclude that the liver fluke is associated with cholinergic and purinergic dysfunctions, which in turn may influence the pathogenesis of the disease. [ABSTRACT FROM AUTHOR]