부산대학교 도서관

Aim of this study was to investigate the effects of the resin monomer BisGMA on the glutathione concentration (monobromobimane assay) and apoptosis (Annexin V/PI-assay) of cultured primary human gingival fibroblasts. Cells were treated for up to 24 h with 0.001–0.25 mm BisGMA to determine growth curves using the DNA stain H33342. Subsequent Annexin V/PI-assays revealed that fibroblasts exposed to concentrations of 0.005–0.01 mm (non-cytotoxic) and 0.05 mm (ED10-concentration) showed no increase of the share of apoptotic cells compared to non-treated controls (5–8%), while 0.1 mm BisGMA (∼ ED50-concentration) caused a significant increase of the percentage of apoptotic cells (50%). Simultaneously to the induction of apoptosis, 0.1 and 0.25 mm of BisGMA caused a significant depletion of the intracellular GSH content after 18 h of incubation. Our results indicate that BisGMA at concentrations >0.1 mm causes an extreme depletion of the intracellular GSH pool as well as apoptosis. [Copyright &y& Elsevier]