학술논문

Screening for and purification of novel self-aggregatable lectins reveal a new functional lectin group in the bark of leguminous trees
Document Type
Article
Source
BBA - General Subjects. Oct2005, Vol. 1726 Issue 1, p21-27. 7p.
Subject
*LEGUMES
*HEMAGGLUTININ
*IMMUNOGLOBULINS
*GLYCOPROTEINS
Language
ISSN
0304-4165
Abstract
Abstract: A solubility–insolubility transition assay was used to screen the bark and stems of seven leguminous trees and plants for self-aggregatable lectins. Novel lectins were found in two trees, Robinia pseudoacacia and Wisteria floribunda, but not in the leguminous plants. The Robinia lectin was isolated from coexisting lectin by combined affinity chromatographies on various sugar adsorbents. The purified lectins proved to be differently glycosylated glycoproteins. One lectin exhibited the remarkable characteristics of self-aggregatable lectins: localization in the bark of legume trees, self-aggregation dissociated by N-acetylglucosamine/mannose, and coexistence with N-acetylgalactosamine/galactose-specific lectins, which are potential endogenous receptors. Self-aggregatable lectins are a functional lectin group that can link enhanced photosynthesis to dissociation of glycoproteins. [Copyright &y& Elsevier]