학술논문
Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription.
Document Type
Article
Author
Source
Subject
*CANCER
*DNA
*CANCER cells
*METHYLATION
*NUCLEIC acids
*
*
*
*
Language
ISSN
1471-2164
Abstract
Background: Cancer is commonly associated with widespread disruption of DNA methylation, chromatin modification and miRNA expression. In this study, we established a robust discovery pipeline to identify epigenetically deregulated miRNAs in cancer. Results: Using an integrative approach that combines primary transcription, genome-wide DNA methylation and H3K9Ac marks with microRNA (miRNA) expression, we identified miRNA genes that were epigenetically modified in cancer. We find miR-205, miR-21, and miR-196b to be epigenetically repressed, and miR-615 epigenetically activated in prostate cancer cells. Conclusions: We show that detecting changes in primary miRNA transcription levels is a valuable method for detection of local epigenetic modifications that are associated with changes in mature miRNA expression. [ABSTRACT FROM AUTHOR]