학술논문
EFFECT OF HYPEROXIA ON INTERLEUKIN-8 EXPRESSION IN PREMATURE VERSUS TERM RABBIT LUNG EXPLANTS.
Document Type
Article
Source
Subject
*CHEMOKINES
*LUNG injuries
*BRONCHOPULMONARY dysplasia
*LUNG diseases
*INTERLEUKIN-8
*IMMUNOHISTOCHEMISTRY
*PROTEINS
*FLUORESCENCE
*
*
*
*
*
*
*
Language
ISSN
0190-2148
Abstract
To examine whether prematurity significantly changes the lung inflammatory response to oxygen, rabbit lung explant cultures were exposed to 95% or 5% oxygen for 24 hours. Interleukin (IL)- 8 protein concentrations from homogenates of the premature lung rose significantly after hyperoxia (6.8 ±1.8 in 5% O2 to 45.7 ±21.3 pg/mg protein in 95% O2) but not in the term lung (15.9 ±6.7 to 20.4 ±4.3 pg/µg protein). There was no change in IL-8 mRNA after hyperoxia in either age group. Preterm lungs demonstrated higher IL-8 levels by fluorescence-activated cell sorting (FACs) analysis and immunohistochemistry. This model may help determine why premature lungs are more susceptible to oxygen-induced disease. [ABSTRACT FROM AUTHOR]