학술논문

Replication and single-cycle delivery of SARS-CoV-2 replicons.
Document Type
Article
Source
Science. 11/26/2021, Vol. 374 Issue 6571, p1099-1106. 8p. 6 Color Photographs.
Subject
*MOLECULAR virology
*CORONAVIRUS diseases
*SARS disease
*YEAST
*GENETICS
Language
ISSN
0036-8075
Abstract
Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization. [ABSTRACT FROM AUTHOR]