학술논문
Genome of the marsupial Monodelphis domestica reveals innovation in non-coding sequences.
Document Type
Article
Author
Mikkelsen, Tarjei S.; Wakefield, Matthew J.; Aken, Bronwen; Amemiya, Chris T.; Chang, Jean L.; Duke, Shannon; Garber, Manuel; Gentles, Andrew J.; Goodstadt, Leo; Heger, Andreas; Jurka, Jerzy; Kamal, Michael; Mauceli, Evan; Searle, Stephen M. J.; Sharpe, Ted; Baker, Michelle L.; Batzer, Mark A.; Benos, Panayiotis V.; Belov, Katherine; Clamp, Michele
Source
Subject
*OPOSSUMS
*NUCLEOTIDE sequence
*GENOMES
*CHROMOSOME analysis
*ANIMAL genome mapping
*X chromosome
*CHROMOSOMAL proteins
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Language
ISSN
0028-0836
Abstract
We report a high-quality draft of the genome sequence of the grey, short-tailed opossum (Monodelphis domestica). As the first metatherian (‘marsupial’) species to be sequenced, the opossum provides a unique perspective on the organization and evolution of mammalian genomes. Distinctive features of the opossum chromosomes provide support for recent theories about genome evolution and function, including a strong influence of biased gene conversion on nucleotide sequence composition, and a relationship between chromosomal characteristics and X chromosome inactivation. Comparison of opossum and eutherian genomes also reveals a sharp difference in evolutionary innovation between protein-coding and non-coding functional elements. True innovation in protein-coding genes seems to be relatively rare, with lineage-specific differences being largely due to diversification and rapid turnover in gene families involved in environmental interactions. In contrast, about 20% of eutherian conserved non-coding elements (CNEs) are recent inventions that postdate the divergence of Eutheria and Metatheria. A substantial proportion of these eutherian-specific CNEs arose from sequence inserted by transposable elements, pointing to transposons as a major creative force in the evolution of mammalian gene regulation. [ABSTRACT FROM AUTHOR]