학술논문
Structural elucidation and antioxidant activity of an arabinogalactan from the leaves of Moringa oleifera.
Document Type
Article
Source
Subject
*MORINGA oleifera
*ARABINOGALACTAN
*GEL permeation chromatography
*RADICAL cations
*MOLECULAR weights
*SEPHAROSE
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Language
ISSN
0141-8130
Abstract
A novel arabinogalactan (MOP-1) was isolated from leaves of Moringa oleifera and was purified by macro-porous resin, DEAE-52 Cellulose and Sepharose 6B gel filtration chromatography. High performance gel permeation chromatography (HPGPC) analysis showed that the molecular weight of MOP-1 was 7.65 × 10 7 Da. Monosaccharide composition analysis indicated that sugar composition is Rha: Ara: Gal in a molar ratio of 1:7.32:12.12. Structural analysis indicated that MOP-1 has a backbone of →1)- β - d -Gal p -(3,4→ with highly branched chains at O-4 position. The branches were composed of →1)- β - d -Gal p -(4→, →1)- α - d -Gal p -(2→, Ara f -(1→, Gal p -(1→. Its antioxidant activities were evaluated by DPPH radical scavenging capacity, 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radical cation scavenging capacity, and ferric-reducing antioxidant power (FRAP). The results indicated MOP-1 exhibited significant antioxidant activities. [ABSTRACT FROM AUTHOR]