학술논문
Chemoattractants for neutrophils lipopolysaccharide-induced inflammatory exudate from rats are not interleukin-8 counterparts by gro-gene-product/melanoma-growth-stimulating-activity-related factors.
Document Type
Article
Author
Source
Subject
*NEUTROPHILS
*ENDOTOXINS
*INTERLEUKIN-8
*MELANOMA
*GROWTH factors
*ION exchange chromatography
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Language
ISSN
0014-2956
Abstract
Potent chemotactic activity for neutrophils was detected in rat inflammatory exudate induced by a subcutaneous injection of lipopolysaccharide in a carboxymethyl-cellulose suspension. We purified and characterized chemoattractants from the exudate by the following procedures: carboxymethyl-Sephadex C-25 ion-exchange chromatography; G3000SW gel-filtration chromatography; preparative reverse-phase high-pressure liquid chromatography; rechromatography on reverse-phase HPLC. Two chemotactic factors were purified and their N-terminal amino acid sequences were determined. One factor was a protein in which the first 20 N-terminal amino acids were identical to those of rat cytokine-induced neutrophil chemoattractant (CINC), a counterpart of human gro/melanoma growth-stimulating activity (MGSA). The other factor was highly similar to mouse macrophage inflammatory protein 2 (MIP-2), Mouse MIP-2, a chemotactic factor for neutrophils, is a member of the interleukin-8 family; however the protein we purified had higher similarity to human gro/MGSA than the human interleukin-8. These results indicate that, in rats, chemotactic factors for neutrophils induced by lipopolysaccharide stimulation are not counterparts of interleukin-8, but we gro/CINC-related peptides. [ABSTRACT FROM AUTHOR]