학술논문
Refinement of an ovine-based immunoglobulin therapy against SARS-CoV-2, with comparison of whole IgG versus F(ab′)2 fragments.
Document Type
Article
Author
Findlay-Wilson, Stephen; Easterbrook, Linda; Smith, Sandra; Pope, Neville; Aldridge, Matthew; Humphries, Gareth; Schuhmann, Holger; Ngabo, Didier; Rayner, Emma; Otter, Ashley; Coleman, Thomas; Hicks, Bethany; Halkerston, Rachel; Apostolakis, Kostis; Taylor, Stephen; Fotheringham, Susan; Horton, Amanda; CanoCejas, Irene; Wand, Matthew; Tree, Julia A.
Source
Subject
*SEROTHERAPY
*SARS-CoV-2
*IMMUNOGLOBULIN G
*SARS-CoV-2 Omicron variant
*RECOMBINANT proteins
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Language
ISSN
2045-2322
Abstract
The development of new therapies against SARS-CoV-2 is required to extend the toolkit of intervention strategies to combat the global pandemic. In this study, hyperimmune plasma from sheep immunised with whole spike SARS-CoV-2 recombinant protein has been used to generate candidate products. In addition to purified IgG, we have refined candidate therapies by removing non-specific IgG via affinity binding along with fragmentation to eliminate the Fc region to create F(ab′)2 fragments. These preparations were evaluated for in vitro activity and demonstrated to be strongly neutralising against a range of SARS-CoV-2 strains, including Omicron B2.2. In addition, their protection against disease manifestations and viral loads were assessed using a hamster SARS-CoV-2 infection model. Results demonstrated protective effects of both IgG and F(ab′)2, with the latter requiring sequential dosing to maintain in vivo activity due to rapid clearance from the circulation. [ABSTRACT FROM AUTHOR]