학술논문
Image fusion of IR and optical microscopy for mapping of biomolecules in tissue.
Document Type
Article
Author
Source
Subject
*MICROSCOPY
*SPATIAL resolution
*HEMATOXYLIN & eosin staining
*BIOMOLECULES
*OPTICAL resolution
*IMAGING systems in chemistry
*IMAGE fusion
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Language
ISSN
0003-2654
Abstract
It is shown that a pixel-level image fusion technique can produce images that combine the spatial resolution of optical microscopy images of haematoxylin and eosin (H&E) stained tissue with the chemical information in Fourier transform infrared (FTIR) images. The fused images show minimal distortion and the higher spatial resolution of the H&E images overcomes the diffraction limit on the spatial resolution of the FTIR images. A consideration of the FTIR spectra of nucleic acids and collagen can explain the changes in contrast between non-cancerous oral epithelium and underlying stroma within fused images formed by combining an H&E stain of oral tissue with FTIR images of the tissue obtained at a number of wavenumbers. [ABSTRACT FROM AUTHOR]