학술논문
Detection in human sera to excreted secreted antigens Toxoplasma gondii by use immunoblot assay of IgG, IgM and IgA from of dot ELISA and.
Document Type
Article
Source
Subject
*IMMUNOGLOBULINS
*ENZYME-linked immunosorbent assay
*ANTIGENS
*TOXOPLASMA gondii
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Language
ISSN
0003-4983
Abstract
The excreted/secreted antigens (ESA) of Toxoplasma gondii are thought to play an important role in the pathogenesis and immune escape of the parasite and the host's immunity to infection with it. In the present study, ESA from peritoneal exudates of infected mice were precipitated with ammonium sulphate (a 40% saturated solution giving the best yield of antigenic components). They were then analysed by immunoblot assay and dot-ELISA using serum samples from 25 patients with toxoplasmosis (19 in the acute phase and six chronic). Three groups of antigens, with molecular masses of 35, 30-32 and 26 kDa, reacted strongly with IgG antibodies, and antigens from two of these groups (30-32 and 26 kDa) also reacted with IgM and IgA. [ABSTRACT FROM AUTHOR]