부산대학교 도서관

Background: Enrofloxacin used considerably on large scale among poultry birds. Detection and monitoring of its residues is vital in the domestic and export market. A simple and rapid LCMS/MS method for screening of Enrofloxacin and its metabolite residues in poultry liver samples was described. Methods: Target analytes were extracted with a solvent combination of 5% phosphoric acid in 50% acetonitrile in water. It was followed by deep freezing for efficient separation of the liquid phase. Later extractants were subjected to centrifugation at 4°C, 10,000 rpm for 20 minutes and supernatant was filtered and injected into LCMS/MS. 0.1% formic acid is used as mobile phase additive for both aqueous and organic mobile phase acetonitrile. Result: Accuracy and precision values of the method are within the acceptable limit of (<5% RSD) accorded by the European commission. Standardized method has been applied to determine the Enrofloxacin and Ciprofloxacin residues in liver samples collected in local markets and found within maximum residue limits. [ABSTRACT FROM AUTHOR]