학술논문

Interactome of the yeast RNA polymerase III transcription machinery constitutes several chromatin modifiers and regulators of the genes transcribed by RNA polymerase II.
Document Type
Article
Source
Gene. Jun2019, Vol. 702, p205-214. 10p.
Subject
*RNA polymerase III
*CHROMATIN
*PROTEIN folding
*DNA damage
*CELL growth
Language
ISSN
0378-1119
Abstract
Eukaryotic transcription is a highly regulated fundamental life process. A large number of regulatory proteins and complexes, many of them with sequence-specific DNA-binding activity are known to influence transcription by RNA polymerase (pol) II with a fine precision. In comparison, only a few regulatory proteins are known for pol III, which transcribes genes encoding small, stable, non-translated RNAs. The pol III transcription is precisely regulated under various stress conditions. We used pol III transcription complex (TC) components TFIIIC (Tfc6), pol III (Rpc128) and TFIIIB (Brf1) as baits and mass spectrometry to identify their potential interactors in vivo. A large interactome constituting chromatin modifiers, regulators and factors of transcription by pol I and pol II supports the possibility of a crosstalk between the three transcription machineries. The association of proteins and complexes involved in various basic life processes like ribogenesis, RNA processing, protein folding and degradation, DNA damage response, replication and transcription underscores the possibility of the pol III TC serving as a signaling hub for communication between the transcription and other cellular physiological activities under normal growth conditions. We also found an equally large number of proteins and complexes interacting with the TC under nutrient starvation condition, of which at least 25% were non-identical under the two conditions. The data reveal the possibility of a large number of signaling cues for pol III transcription against adverse conditions, necessary for an efficient co-ordination of various cellular functions. • Many proteins interact with yeast RNA polymerase III transcription complex (TC). • The interactome consists of primarily the proteins involved in basic life processes. • Pol III TC interacts with many cytoplasmic, stress and chromatin-related proteins. • DNA repair and pol II transcription factors, including Paf1 interact with pol III TC. • At least 25% of the TC-interacting proteins are unique for active or repressed states. [ABSTRACT FROM AUTHOR]