부산대학교 도서관

Abstract: Background: IL‐35 modulates immune and inflammatory responses during infections. Here, we investigated IL‐35 levels and a single nucleotide polymorphism, rs3761548, in FOXP3 gene in Helicobacter pylori‐infected patients with peptic ulcer (PU), to clarify possible associations. Materials and Methods: This study includes 100 H. pylori‐infected PU patients, 100 H. pylori‐infected asymptomatic subjects (AS), and 100 noninfected healthy subjects (NHSs). Serum IL‐35 levels and the genotyping were determined using ELISA and RFLP‐PCR methods, respectively. Results: In PU patients, the IL‐35 levels were lower than AS and NHS groups (P < .001). The IL‐35 levels in CagA+ H. pylori‐infected participants from PU and AS groups were lower than individuals infected with CagA‐ strains (P < .02 and P < .04, respectively). Women had higher IL‐35 levels than men among PU, AS, and NHS groups (P < .0001). In PU patients, AA genotype and A allele at rs3761548 were more frequent than total healthy subjects (AS + NHS groups) and associated with an increased PU risk (AA genotype: OR = 5.51, P < .0001; A allele: OR = 3.857, P < .002). In PU and AS groups, IL‐35 levels were lower in subjects displaying AA genotype or A allele than subjects displaying CC genotype or C allele, respectively (P < .0001 and P < .03 for PU patients; P < .001 and P < .02 for AS group, respectively). Conclusions: Decreased IL‐35 levels could be involved in PU development in H. pylori‐infected individuals. IL‐35 levels are affected by CagA status of H. pylori, participants gender, and genetic variations at rs3761548. The AA genotype and A allele at rs3761548 could represent a risk factor for PU development. [ABSTRACT FROM AUTHOR]