학술논문
Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2.
Document Type
Journal Article
Author
Banerjee, Arinjay; Nasir, Jalees A.; Budylowski, Patrick; Yip, Lily; Aftanas, Patryk; Christie, Natasha; Ghalami, Ayoob; Baid, Kaushal; Raphenya, Amogelang R.; Hirota, Jeremy A.; Miller, Matthew S.; McGeer, Allison J.; Ostrowski, Mario; Kozak, Robert A.; McArthur, Andrew G.; Mossman, Karen; Mubareka, Samira
Source
Subject
*COVID-19
*COVID-19 pandemic
*SINGLE nucleotide polymorphisms
*VIRAL pneumonia
*RESEARCH
*MONONUCLEAR leukocytes
*DNA
*RESEARCH methodology
*GENETIC polymorphisms
*EVALUATION research
*MEDICAL cooperation
*COMPARATIVE studies
*GENOTYPES
*EPIDEMICS
*RESEARCH funding
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Language
ISSN
1080-6040
Abstract
Since its emergence in Wuhan, China, in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected ≈6 million persons worldwide. As SARS-CoV-2 spreads across the planet, we explored the range of human cells that can be infected by this virus. We isolated SARS-CoV-2 from 2 infected patients in Toronto, Canada; determined the genomic sequences; and identified single-nucleotide changes in representative populations of our virus stocks. We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. As SARS-CoV-2 continues to spread globally, it is essential to monitor single-nucleotide polymorphisms in the virus and to continue to isolate circulating viruses to determine viral genotype and phenotype by using in vitro and in vivo infection models. [ABSTRACT FROM AUTHOR]