부산대학교 도서관

Metabolism is directly and indirectly fine-tuned by a complex web of interacting regulatory mechanisms that fall into two major classes. On the one hand, the expression level of the catalyzing enzyme sets the maximal theoretical flux level (i.e., the net rate of the reaction) for each enzyme-controlled reaction. On the other hand, metabolic regulation controls the metabolic flux through the interactions of metabolites (substrates, cofactors, allosteric modulators) with the responsible enzyme. High-throughput data, such as metabolomics and transcriptomics data, if analyzed separately, do not accurately characterize the hierarchical regulation of metabolism outlined above. They must be integrated to disassemble the interdependence between different regulatory layers controlling metabolism. To this aim, we propose INTEGRATE, a computational pipeline that integrates metabolomics and transcriptomics data, using constraint-based stoichiometric metabolic models as a scaffold. We compute differential reaction expression from transcriptomics data and use constraint-based modeling to predict if the differential expression of metabolic enzymes directly originates differences in metabolic fluxes. In parallel, we use metabolomics to predict how differences in substrate availability translate into differences in metabolic fluxes. We discriminate fluxes regulated at the metabolic and/or gene expression level by intersecting these two output datasets. We demonstrate the pipeline using a set of immortalized normal and cancer breast cell lines. In a clinical setting, knowing the regulatory level at which a given metabolic reaction is controlled will be valuable to inform targeted, truly personalized therapies in cancer patients. Author summary: The study of metabolism and its regulation finds increasing application in various fields, including biotransformations, wellness, and health. Metabolism can be studied using post-genomic technologies. Transcriptomics, proteomics and metabolomics provide snapshots of transcripts, enzyme levels and metabolites in specific physio-pathological conditions. In the health field, the transcriptome and, more recently, the metabolome have been broadly profiled at the pre-clinical and clinical levels, while only more recently proteomic studies profiling metabolic enzymes are becoming available. However, the informative power of single omic technologies is inadequate since metabolism regulation involves a complex interplay of regulatory steps. Gene expression regulates metabolism by setting the upper level of metabolic enzymes, whereas the interaction of metabolites with metabolic enzymes directly auto-regulates metabolism. Therefore, there is a need for methods that integrate multiple data sources. We present INTEGRATE, a computational pipeline that captures dynamic features from the static snapshots provided by transcriptomics and metabolomics data. Through integration in a steady-state metabolic model, the pipeline predicts which reactions are controlled purely by metabolic control rather than by gene expression or a combination of the two. This knowledge is crucial in a clinical setting to develop personalized therapies in patients of multifactorial diseases, such as cancer. Besides cancer, INTEGRATE can be applied to different fields where metabolism plays a driving role. [ABSTRACT FROM AUTHOR]