학술논문
Lability of Stationary and Time-Resolved Optical Properties of the Conformationally Locked CFP Chromophore Derivative.
Document Type
Article
Author
Source
Subject
*OPTICAL properties
*FLUORESCENT proteins
*TIME-resolved spectroscopy
*TERTIARY amines
*PROTEIN engineering
*FLUORIMETRY
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Language
ISSN
1068-1620
Abstract
The solvatochromic behavior of a model compound mimicking the chromophore of cyan fluorescent protein (CFP) in the spectral and time domains has been studied. Analysis of the time-resolved fluorescence signal, carried out in 40 solvents belonging to different classes, showed that the lipophilic environment promotes an increase, while aromatic solvents and tertiary amines, a decrease in the fluorescence lifetime of the chromophore. Based on this study, the possible rational design of CFP-like proteins was proposed. [ABSTRACT FROM AUTHOR]