학술논문
Pneumococcal carriage in unvaccinated children at the time of vaccine implementation into the national immunization program in Poland.
Document Type
Article
Author
Wróbel-Pawelczyk, Izabela; Ronkiewicz, Patrycja; Wanke-Rytt, Monika; Rykowska, Dominika; Górska-Kot, Aneta; Włodkowska, Katarzyna; Topczewska-Cabanek, Agnieszka; Jackowska, Teresa; Chruszcz, Joanna; Marchut, Walentyna; Mastalerz-Migas, Agnieszka; Korzeniewski, Krzysztof; GIL Study Team; Gastoł, Karolina; Gromek, Marta; Jankowska-Sasin, Katarzyna; Karpierz, Katarzyna; Okarska-Napierała, Magdalena; Pokorna-Kałwak, Dagmara; Polit, Agata
Source
Subject
*VACCINATION of children
*VACCINATION
*VACCINATION status
*DAY care centers
*IMMUNIZATION
*
*
*
*
Language
ISSN
2045-2322
Abstract
We investigated pneumococcal carriage among unvaccinated children under five years of age at a time when the conjugate polysaccharide vaccine (PCV) was introduced in Poland into the national immunization program (NIP). Paired nasopharyngeal swab (NPS) and saliva samples collected between 2016 and 2020 from n = 394 children were tested with conventional culture and using qPCR. The carriage rate detected by culture was 25.4% (97 of 394), by qPCR 39.1% (155 of 394), and 40.1% (158 of 394) overall. The risk of carriage was significantly elevated among day care center attendees, and during autumn/winter months. Among isolates cultured, the most common serotypes were: 23A, 6B, 15BC, 10A, 11A. The coverage of PCV10 and PCV13 was 23.2% (23 of 99) and 26.3% (26 of 99), respectively. Application of qPCR lead to detection of 168 serotype carriage events, with serogroups 15, 6, 9 and serotype 23A most commonly detected. Although the highest number of carriers was identified by testing NPS with qPCR, saliva significantly contributed to the overall number of detected carriers. Co-carriage of multiple serotypes was detected in 25.3% (40 of 158) of carriers. The results of this study represent a baseline for the future surveillance of effects of pneumococcal vaccines in NIP in Poland. [ABSTRACT FROM AUTHOR]