학술논문
Purification of human epidermal growth factor (urogastrone) from urine.
Document Type
Article
Author
Source
Subject
*EPIDERMAL growth factor
*GROWTH factors
*URINE
*BODY fluids
*CHROMATOGRAPHIC analysis
*AMINO acids
*
*
*
*
*
Language
ISSN
0014-2956
Abstract
Human epidermal growth factor has been isolated from a concentrated chromatographic eluate of human urine. The purification method u utilizes six chromatographic steps including adsorption to aminoethylcellulose (AE-11), gel filtration on Sephadex G-50, carboxymethylcellulose (CM-52) chromatography, ion-exchange HPLC and reverse-phase HPLC. The final product appears homogeneous and identical to pure γ-urogastrone when analyzed by polyacrylamide gel electrophoresis and reverse-phase HPLC using two eluent systems. The yield of the method described above allowed the development of a sensitive radioimmunoassay system for this growth factor. [ABSTRACT FROM AUTHOR]