부산대학교 도서관

Electrospun scaffolds are a versatile biomaterial platform to mimic fibrillar structure of native tissues extracellular matrix, and facilitate the incorporation of biomolecules for regenerative therapies. Self-assembling peptide P 11 -4 has emerged as a promising strategy to induce mineralization; however, P 11 -4 application has been mostly addressed for early caries lesions repair on dental enamel. Here, to investigate P 11 -4′s efficacy on bone regeneration, polymeric electrospun scaffolds were developed, and then distinct concentrations of P 11 -4 were physically adsorbed on the scaffolds. P 11 -4-laden and pristine (P 11 -4-free) electrospun scaffolds were immersed in simulated body fluid and mineral precipitation identified by SEM. Functional groups and crystalline phases were analyzed by FTIR and XRD, respectively. Cytocompatibility, mineralization, and gene expression assays were conducted using stem cells from human exfoliated deciduous teeth. To investigate P 11 -4-laden scaffolds potential to induce in vivo mineralization, an established rat calvaria critical-size defect model was used. Results. We successfully synthesized nanofibrous (∼ 500 nm fiber diameter) scaffolds and observed that functionalization with P 11 -4 did not affect the fibers' diameter. SEM images indicated mineral precipitation, while FTIR and XRD confirmed apatite-like formation and crystallization for P 11 -4-laden scaffolds. In addition, P 11 -4-laden scaffolds were cytocompatible, highly stimulated cell-mediated mineral deposition, and upregulated the expression of mineralization-related genes compared to pristine scaffolds. P 11 -4-laden scaffolds led to enhanced in vivo bone regeneration after 8 weeks compared to pristine PCL. Electrospun scaffolds functionalized with P 11 -4 are a promising strategy for inducing mineralized tissues regeneration in the craniomaxillofacial complex. [Display omitted] • Self-assembly peptide P 11 -4-laden scaffolds induces mineral precipitation. • P 11 -4-laden scaffolds stimulate stem cells to mineral deposition. • P 11 -4-laden scaffolds upregulate osteogenic markers. • P 11 -4-laden scaffolds induces bone formation in vivo. [ABSTRACT FROM AUTHOR]