학술논문
Gastroprotective effects and structural characterization of a pectic fraction isolated from Artemisia campestris subsp maritima.
Document Type
Article
Author
Source
Subject
*ARTEMISIA
*PECTIC enzymes
*GALACTURONAN
*CHEMICAL structure
*ARABINOGALACTAN
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Language
ISSN
0141-8130
Abstract
The aim of this study was to investigate the chemical structure and biological activity of a pectic fraction isolated from the aerial parts of A. campestris L . subsp. maritima Arcangeli. The chemical and spectroscopic analyses of the pectic fraction (ACP-E10) demonstrated that ACP-E10 was composed of homogalacturonan (HG) (60%) and rhamnogalacturonan-I (RG-I) (29%) regions. Side chains of the RG-I included mainly branched arabinans and type II arabinogalactans (AG-II). The molar mass of ACP-E10 determined by HPSEC-MALLS was 16,600 g/mol. ACP-E10 was evaluated for its gastroprotective effect against ethanol-induced gastric lesions in rats. Oral pretreatment of animals with ACP-E10 (0.3, 3 and 30 mg/kg) significantly reduced gastric lesions by 77 ± 7.9%, 55 ± 11.1% and 65 ± 11.8%. ACP-E10 also maintained mucus and glutathione (GSH) contents in the gastric mucosa. In addition, ACP-E10 demonstrated antioxidant activity in vitro by the DPPH assay. These results demonstrated that the pectin from A. campestris had significant gastroprotective effects in vivo , which were likely attributable to their capacity to increase the protective defenses of gastric mucosa. [ABSTRACT FROM AUTHOR]