부산대학교 도서관

Objectives: Early chimerism analysis is important to assess engraftment in allogeneic hematopoietic stem cell transplantations. Methods: We retrospectively investigated the impact of T‐cell chimerism at day 30 in bone marrow on acute graft‐versus‐host disease (aGVHD), relapse, and overall survival in 142 adult allo‐transplanted patients. Results: The majority of patients (89%) received myeloablative conditioning and 90% have undergone T‐cell replete donor graft. At day 30, 103 patients showed T‐complete chimerism with prevalence in haploidentical transplants, whereas 39 cases had CD3+ mixed chimerism, including 30 patients transplanted with HLA identical donors, and 21 with T‐cell donors<90%. T‐cell chimerism at day 30 was weakly inversely related to aGVHD grades II–IV (p =.078) with no cases of grades III–IV aGVHD in patients with CD3+ <95%. Mixed T‐cell chimerism did not impact on relapse (p =.448) and five of the seven patients who relapsed had T‐cell chimerism ≤90%. Older age and active disease at transplant had a statistically significant negative effect on overall survival (p =.01 and p =.0001, respectively), whereas mixed CD3+ chimerism did not. Conclusions: T lymphocyte chimerism analysis at day +30 in bone marrow could identify allo‐transplanted patients at major risk of aGVHD grades III–IV (CD3+ donors >95%) mainly post‐myeloablative conditioning regimen. [ABSTRACT FROM AUTHOR]