학술논문
Ongoing Y-chromosome instability defines sub-clonal variants in radiation-induced leukaemias in the mouse.
Document Type
Article
Author
Source
Subject
*LEUKEMIA
*PHYSIOLOGICAL effects of radiation
*Y chromosome abnormalities
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Language
ISSN
0955-3002
Abstract
Forty primary leukaemias that arose in vivo as a consequence of 3 Gy X-irradiation of inbred mouse strains were analysed for Y-chromosome aberrations by conventional cytogenetics and fluorescent in situ hybridization (FISH). Compared with control mice which were X-irradiated but which exhibited no overt signs of leukaemia, the loss and gain of Y-chromosomes in leukaemic spleen cells defined subclonal variants in the radiation-induced haempoietic malignancies that arose in CBA/H, DBA/2 and (C57BL/6 DBA/2)F1 mice. This Y-chromosome instability was significantly higher than that observed in spleen cells of age-matched (or older) irradiated control mice that had not developed overt leukaemia. The detection of Y-chromosome aberrations is considered in the context of the high numbers of potential gene regulatory sequences in the murine Y-chromosome and the potential for the insertional activation of cellular genes during multi-stage radiation leukaemogenesis. [ABSTRACT FROM AUTHOR]