학술논문

Cytogenetics of Alismataceae and Limnocharitaceae: CMA/DAPI banding and 45S rDNA sites.
Document Type
Article
Source
Plant Systematics & Evolution. May2010, Vol. 286 Issue 3/4, p199-208. 10p. 3 Color Photographs, 1 Diagram, 1 Chart.
Subject
*PLANT cytogenetics
*IN situ hybridization
*CHROMATIN
*CELL nuclei
*PLANT species
Language
ISSN
0378-2697
Abstract
Species belonging to the Alismataceae ( Echinodorus) and Limnocharitaceae ( Hydrocleys and Limnocharis) families were analysed by banding with CMA/DAPI fluorochromes, C/CMA/DAPI banding, and in situ hybridization (FISH) with probes that recognise 45S rDNA. All species of Echinodorus presented 2 n = 22, but only in E. lanceolatus were DAPI+ telomeric bands in seven chromosome pairs observed. A bimodal karyotype and GC-rich heterochromatin preferably located in two smaller acrocentric pairs that generally corresponded to the number of sites of 45S rDNA. A similar pattern of bands was observed in both Limnocharis species (2 n = 20), but the two differed with respect to 45S rDNA, with L. laforestii showing only two sites. Hydrocleys nymphoides and H. martii had a chromosome number of 2 n = 16, but the position of the GC-rich heterochromatin associated with the satellite differed among chromosomal types. In this work, the cytotaxonomic implications of these patterns are discussed and correlated with previous data from the literature. [ABSTRACT FROM AUTHOR]