학술논문
Sharper low-power STED nanoscopy by time gating.
Document Type
Article
Author
Source
Subject
*FLUORESCENCE
*MICROSCOPY
*MOLECULAR interactions
*SIGNAL-to-noise ratio
*CELL membranes
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Language
ISSN
1548-7091
Abstract
The article focuses on the enhancement of fluorescence in low power continuous-wave stimulated emission depletion (CW-STED) microscopy, by applying pulsed excitation with time-gated detection imaging of living cells. Topics include detection of nanoscale molecular interactions by fluorescence, reduction in signal-to-noise ratio and comparison of keratin cell by CW-STED and gate-STED (g-STED). It further mentions the detection of lipid molecules in the plasma membrane of living cells.