학술논문
Designing chromatic optical retarder stacks for segmented next‐generation easySTED phase plates.
Document Type
Article
Author
Source
Subject
*MOLECULAR beams
*LIGHT sources
*OPTICAL elements
*LASER beams
*PASSIVE optical networks
*FLUORESCENCE
*EULER-Bernoulli beam theory
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Language
ISSN
0022-2720
Abstract
Fluorescence nanoscopy methods based on the RESOLFT principle, such as beam‐scanning STED nanoscopy, require the co‐alignment of optical beams for molecular state (on/off) switching and fluorescence excitation. The complexity and stability of the beam alignment can be drastically simplified and improved by using a single‐mode fibre as the sole light source for all required laser beams. This in turn then requires a chromatic optical element for shaping the off‐switching beam into a focal‐plane donut while simultaneously leaving the focal intensity distributions at other wavelengths shaped as regular focal spots. Here we describe novel designs of such so‐called 'easySTED phase plates' and provide a rationale how to find the desired spectral signature for combinations of multiple wavelengths. [ABSTRACT FROM AUTHOR]