학술논문
Expanding NIST Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles.
Document Type
Article
Source
Subject
*FLOW cytometry
*FLUORESCENCE
*MICROSPHERES
*CALIBRATION
*PARTICLES
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Language
ISSN
1996-1944
Abstract
The National Institute of Standards and Technology (NIST), the National Institutes of Health (NIH) and other industry stakeholders have been working together to enable fluorescence intensities of flow cytometer calibration beads to be assigned quantitative equivalent reference fluorophore (ERF) values with high accuracy and precision. The ultimate goal of this effort is to accurately quantify the number of antibodies bound to individual living cells. The expansion of this effort to assign ERF values to more than 50 fluorescence channels and particles with diameters ranging from 10 μm down to 80 nm is reported here. [ABSTRACT FROM AUTHOR]