학술논문
SARS‐CoV‐2 infection impacts carbon metabolism and depends on glutamine for replication in Syrian hamster astrocytes.
Document Type
Article
Author
de Oliveira, Lilian Gomes; de Souza Angelo, Yan; Yamamoto, Pedro; Carregari, Victor Corasolla; Crunfli, Fernanda; Reis‐de‐Oliveira, Guilherme; Costa, Lícia; Vendramini, Pedro Henrique; Duque, Érica Almeida; dos Santos, Nilton Barreto; Firmino, Egidi Mayara; Paiva, Isadora Marques; Almeida, Glaucia Maria; Sebollela, Adriano; Polonio, Carolina Manganeli; Zanluqui, Nagela Ghabdan; de Oliveira, Marília Garcia; da Silva, Patrick; Davanzo, Gustavo Gastão; Ayupe, Marina Caçador
Source
Subject
*GOLDEN hamster
*CARBON metabolism
*SARS-CoV-2
*GLUTAMINE
*KETOGLUTARIC acids
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Language
ISSN
0022-3042
Abstract
COVID‐19 causes more than million deaths worldwide. Although much is understood about the immunopathogenesis of the lung disease, a lot remains to be known on the neurological impact of COVID‐19. Here, we evaluated immunometabolic changes using astrocytes in vitro and dissected brain areas of SARS‐CoV‐2 infected Syrian hamsters. We show that SARS‐CoV‐2 alters proteins of carbon metabolism, glycolysis, and synaptic transmission, many of which are altered in neurological diseases. Real‐time respirometry evidenced hyperactivation of glycolysis, further confirmed by metabolomics, with intense consumption of glucose, pyruvate, glutamine, and alpha ketoglutarate. Consistent with glutamine reduction, the blockade of glutaminolysis impaired viral replication and inflammatory response in vitro. SARS‐CoV‐2 was detected in vivo in hippocampus, cortex, and olfactory bulb of intranasally infected animals. Our data evidence an imbalance in important metabolic molecules and neurotransmitters in infected astrocytes. We suggest this may correlate with the neurological impairment observed during COVID‐19, as memory loss, confusion, and cognitive impairment. [ABSTRACT FROM AUTHOR]