학술논문
Fluid shear stress enhances proliferation of breast cancer cells via downregulation of the c-subunit of the F1FO ATP synthase.
Document Type
Article
Author
Source
Subject
*ADENOSINE triphosphatase
*CANCER cell proliferation
*SHEARING force
*METASTATIC breast cancer
*CANCER cell migration
*CANCER cells
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Language
ISSN
0006-291X
Abstract
The presence of circulating cancer cells in the bloodstream is positively correlated with metastasis. We hypothesize that fluid shear stress (FSS) occurring during circulation alters mitochondrial function, enhancing metastatic behaviors of cancer cells. MCF7 and MDA-MB-231 human breast cancer cells subjected to FSS exponentially increased proliferation. Notably, FSS-treated cells consumed more oxygen but were resistant to uncoupler-mediated ATP loss. We found that exposure to FSS downregulated the F 1 F O ATP synthase c-subunit and overexpression of the c-subunit arrested cancer cell migration. Approaches that regulate c-subunit abundance may reduce the likelihood of breast cancer metastasis. • Fluid shear stress (FSS) alters mitochondrial energy metabolism, increasing the proliferation of breast cancer cells. • FSS decreases protein levels of the F 1 F O ATP synthase c-subunit in breast cancer cells. • Overexpression of the c-subunit slows the migration of breast cancer cells. [ABSTRACT FROM AUTHOR]