학술논문
T-cell specific upregulation of Sema4A as risk factor for autoimmunity in systemic lupus erythematosus and rheumatoid arthritis.
Document Type
Article
Author
Cavalcanti, Catarina Addobbati Jordão; Germoglio, Vanessa; de Azevêdo Silva, Jaqueline; Glesse, Nadine; Vianna, Priscila; Cechim, Giovana; Monticielo, Odirlei Andre; Xavier, Ricardo Machado; Brenol, João Carlos Tavares; Brenol, Claiton Viegas; Fragoso, Thiago Sotero; Barbosa, Alexandre Domingues; Duarte, Ângela Luiza Branco Pinto; Oliveira, Renê Donizeti Ribeiro; Louzada-Júnior, Paulo; Donadi, Eduardo Antônio; Chies, José Artur Bogo; Crovella, Sergio; Sandrin-Garcia, Paula
Source
Subject
*RHEUMATOID arthritis
*AUTOIMMUNITY
*T cells
*SYSTEMIC lupus erythematosus
*BLOOD cells
*STATISTICAL power analysis
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Language
ISSN
0891-6934
Abstract
The aim of the present study was to evaluate the impact of SEMA4A genetic variants on expression of sema4A protein and its relation to autoimmunity development in Systemic Lupus Erythematosus and Rheumatoid Arthritis patients. A total of 541 SLE patients, 390 RA patients and 607 healthy individuals were genotyped. We also assessed SEMA4A mRNA expression from whole blood cells and the in vitro protein production from resting and activated T lymphocytes as well as mature dendritic cells from healthy individuals stratified according to their genotypes for SLE/RA associated SEMA4A variants. Our results showed that T/T genotype for rs3738581 SNP is associated with both RA and SLE development (p =.000053, OR = 2.35; p =.0019, OR = 2.07, respectively; statistical power = 100%) and also to an increased in vitro sema4A production in active T lymphocytes. Our findings are indicative of a T cell-specific upregulation of sema4A in the presence of T/T genotype, being a risk factor for SLE and RA. [ABSTRACT FROM AUTHOR]