학술논문
Skeletal muscle and erythrocyte redox status is associated with dietary cysteine intake and physical fitness in healthy young physically active men.
Document Type
Article
Author
Papanikolaou, Konstantinos; Jamurtas, Athanasios Z.; Poulios, Athanasios; Tsimeas, Panagiotis; Draganidis, Dimitrios; Margaritelis, Nikos V.; Baloyiannis, Ioannis; Papadopoulos, Constantinos; Sovatzidis, Apostolos; Deli, Chariklia K.; Rosvoglou, Anastasia; Georgakouli, Kalliopi; Tzatzakis, Theofanis; Nikolaidis, Michalis G.; Fatouros, Ioannis G.
Source
Subject
*CYSTEINE metabolism
*ERYTHROCYTE metabolism
*BODY composition
*GLUTATHIONE
*SKELETAL muscle
*FOOD consumption
*CARDIOPULMONARY fitness
*MEN
*PHYSICAL fitness
*ANTIOXIDANTS
*DIET
*PHYSICAL activity
*MUSCLE strength
*EXERCISE intensity
*RESEARCH funding
*ATHLETIC ability
*SECONDARY analysis
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Language
ISSN
1436-6207
Abstract
Purpose: To investigate the association between redox status in erythrocytes and skeletal muscle with dietary nutrient intake and markers of physical fitness and habitual physical activity (PA). Methods: Forty-five young physically active men were assessed for body composition, dietary nutrient intake, muscle strength, cardiorespiratory capacity and habitual PA. Blood and muscle samples were collected to estimate selected redox biomarkers. Partial correlation analysis was used to evaluate the independent relationship of each factor with redox biomarkers. Results: Dietary cysteine intake was positively correlated (p < 0.001) with both erythrocyte (r = 0.697) and muscle GSH (0.654, p < 0.001), erythrocyte reduced/oxidized glutathione ratio (GSH/GSSG) (r = 0.530, p = 0.001) and glutathione reductase (GR) activity (r = 0.352, p = 0.030) and inversely correlated with erythrocyte protein carbonyls (PC) levels (r = − 0.325; p = 0.046). Knee extensors eccentric peak torque was positively correlated with GR activity (r = 0.355; p = 0.031) while, one-repetition maximum in back squat exercise was positively correlated with erythrocyte GSH/GSSG ratio (r = 0.401; p = 0.014) and inversely correlated with erythrocyte GSSG and PC (r = − 0.441, p = 0.006; r = − 0.413, p = 0.011 respectively). Glutathione peroxidase (GPx) activity was positively correlated with step count (r = 0.520; p < 0.001), light (r = 0.406; p = 0.008), moderate (r = 0.417; p = 0.006), moderate-to-vigorous (r = 0.475; p = 0.001), vigorous (r = 0.352; p = 0.022) and very vigorous (r = 0.326; p = 0.035) PA. Muscle GSSG inversely correlated with light PA (r = − 0.353; p = 0.022). Conclusion: These results indicate that dietary cysteine intake may be a critical element for the regulation of glutathione metabolism and redox status in two different tissues pinpointing the independent significance of cysteine for optimal redox regulation. Musculoskeletal fitness and PA levels may be predictors of skeletal muscle, but not erythrocyte, antioxidant capacity. Trial registration: Registry: ClinicalTrials.gov, identifier: NCT03711838, date of registration: October 19, 2018. [ABSTRACT FROM AUTHOR]